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fcc co-ordinating director mark howell said in his letter that the united states center for consumer rights statement was a setback in efforts to.The expression of human fibrillin-1 in vascular smooth muscle cells requires interaction with the alpha smooth muscle actin cytoskeleton.
Fibrillin-1 is a major component of the extracellular microfibril scaffold of the extracellular matrix of the vasculature. In tissues, fibrillin-1 is expressed in both mesenchymal and differentiated vascular smooth muscle cells. During development of the vasculature, fibrillin-1 expression increases during differentiation of the myocardium and is induced by the cardiotrophin-1 (CT-1) cytokine. Here we show that in the differentiated rat aortic smooth muscle cell line A10, in which CT-1 is expressed constitutively, expression of fibrillin-1 is maintained by mitogenic stimulation with fetal bovine serum or platelet-derived growth factor-BB. In contrast, in A10 cells stimulated with vascular smooth muscle growth factor (VSMC-GF), expression of fibrillin-1 is decreased. Co-immunoprecipitation studies demonstrate that in A10 cells treated with platelet-derived growth factor-BB, fibrillin-1 interacts with alpha smooth muscle actin. However, in growth factor-stimulated A10 cells, co-immunoprecipitation with anti-fibrillin-1 antibody did not identify the interaction with alpha smooth muscle actin. Furthermore, the inhibition of alpha smooth muscle actin gene expression by antisense oligonucleotides in growth factor-stimulated A10 cells inhibited the expression of fibrillin-1. Thus, vascular smooth muscle cells can express fibrillin-1 and form part of the extracellular matrix. However, the process requires the alpha smooth muscle actin cytoskeleton.John McCain today endorsed President Barack Obama in the days before the November 8 election.